DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP). Academic Article

abstract

• The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.

authors

• Suzuki, Masako

published proceedings

• Methods

author list (cited authors)

• Suzuki, M., & Greally, J. M.

citation count

• 25

publication date

• November 2010

publisher

• Elsevier  Publisher

keywords

• Animals
• DNA
• DNA Methylation
• Deoxyribonuclease Hpaii
• Humans
• Polymerase Chain Reaction

Digital Object Identifier (DOI)

• 10.1016/j.ymeth.2010.04.013

start page

• 218

end page

• 222

volume

• 52

issue

• 3

URL

• http://dx.doi.org/10.1016/j.ymeth.2010.04.013