Optimized design and data analysis of tag-based cytosine methylation assays. Academic Article

abstract

• Using the type III restriction-modification enzyme EcoP15I, we isolated sequences flanking sites digested by the methylation-sensitive HpaII enzyme or its methylation-insensitive MspI isoschizomer for massively parallel sequencing. A novel data transformation allows us to normalise HpaII by MspI counts, resulting in more accurate quantification of methylation at >1.8 million loci in the human genome. This HELP-tagging assay is not sensitive to sequence polymorphism or base composition and allows exploration of both CG-rich and depleted genomic contexts.

authors

• Suzuki, Masako

published proceedings

• Genome Biol

altmetric score

• 1

author list (cited authors)

• Suzuki, M., Jing, Q., Lia, D., Pascual, M., McLellan, A., & Greally, J. M.

citation count

• 77

complete list of authors

• Suzuki, Masako||Jing, Qiang||Lia, Daniel||Pascual, Marién||McLellan, Andrew||Greally, John M

publication date

• January 2010

publisher

• Springer Nature  Publisher

published in

• n1474-760XISSN  Journal

keywords

• Base Sequence
• Cells, Cultured
• Cytosine
• DNA Methylation
• Dna-cytosine Methylases
• Genome, Human
• Humans
• Polymorphism, Genetic
• Sequence Analysis, DNA
• Site-specific Dna-methyltransferase (adenine-specific)

Digital Object Identifier (DOI)

• 10.1186/gb-2010-11-4-r36

start page

• R36

end page

• R36

volume

• 11

issue

• 4

URL

• http://dx.doi.org/10.1186/gb-2010-11-4-r36