Optimized design and data analysis of tag-based cytosine methylation assays. Academic Article uri icon


  • Using the type III restriction-modification enzyme EcoP15I, we isolated sequences flanking sites digested by the methylation-sensitive HpaII enzyme or its methylation-insensitive MspI isoschizomer for massively parallel sequencing. A novel data transformation allows us to normalise HpaII by MspI counts, resulting in more accurate quantification of methylation at >1.8 million loci in the human genome. This HELP-tagging assay is not sensitive to sequence polymorphism or base composition and allows exploration of both CG-rich and depleted genomic contexts.

published proceedings

  • Genome Biol

altmetric score

  • 1

author list (cited authors)

  • Suzuki, M., Jing, Q., Lia, D., Pascual, M., McLellan, A., & Greally, J. M.

citation count

  • 77

complete list of authors

  • Suzuki, Masako||Jing, Qiang||Lia, Daniel||Pascual, MariĆ©n||McLellan, Andrew||Greally, John M

publication date

  • January 2010