Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts. Academic Article uri icon


  • Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research.

published proceedings

  • Stem Cells

altmetric score

  • 0.5

author list (cited authors)

  • Wakayama, S., Jakt, M. L., Suzuki, M., Araki, R., Hikichi, T., Kishigami, S., ... Wakayama, T.

citation count

  • 148

publication date

  • September 2006