Shedding light on ORAI1 channel with genetic code expansion.

abstract

• Genetic code expansion technology has been widely applied to control protein activity and biological systems by taking advantage of an amber stop codon suppressor tRNA and orthogonal aminoacyl-tRNA synthetase pair. With this chemical biology approach, Maltan etal. incorporated photocrosslinking unnatural amino acids (UAAs) into the transmembrane domains of ORAI1 to enable UV light-inducible calcium influx across the plasma membrane, mechanistic interrogation of the calcium release-activated calcium (CRAC) channel at the single amino acid level, and remote control of downstream calcium-modulated signaling in mammalian cells.

authors

• Zhou, Yubin

published proceedings

• Cell Calcium

altmetric score

• 2

author list (cited authors)

• Ali, S., Ma, G., & Zhou, Y.

citation count

• 0

complete list of authors

• Ali, Sher||Ma, Guolin||Zhou, Yubin

publication date

• May 2023

publisher

• Elsevier  Publisher

keywords

• Calcium Signaling
• Crac
• Genetic Code Expansion
• Ion Channel
• Nfat
• Optogenetics
• Orai1
• Photopharmacology
• STIM1
• Unnatural Amino Acid

Digital Object Identifier (DOI)

• 10.1016/j.ceca.2023.102755

start page

• 102755

end page

• 102755

volume

• 113

URL

• http://dx.doi.org/10.1016/j.ceca.2023.102755