Protein kinetics in callipyge lambs. Academic Article uri icon


  • The objectives for this experiment were to determine the effect of the callipyge phenotype on protein kinetics. We studied callipyge and normal lambs (n = 37) at 5, 8, and 11 wk of age (n = 4 to 7/ group) to determine how protein kinetics are altered by this trait. Total protein, DNA, and RNA and calpastatin activity were measured in five skeletal muscles and in the heart, kidneys, and liver, and protein accretion rates were calculated. At 8 wk, the fractional synthesis rates of proteins in these tissues were measured in vivo using a primed, continuous 8-h infusion of [2H5]phenylalanine. Fractional rates of protein degradation were estimated by differences. At 5 wk of age, muscle weights, protein mass, protein:DNA, RNA:DNA, and calpastatin activity were higher (P < .05) for callipyge, and protein mass differences continued to increase through 11 wk. At 8 wk, fractional rates of protein synthesis and degradation were lower (P < .05) in callipyge than in normal lambs. The organs of callipyge lambs exhibited reduced growth at 11 wk. Thus, enhanced muscle growth seems to be maintained in callipyge lambs by reduced protein degradation rather than increased protein synthesis. However, we cannot exclude the possibility that the initial onset of the callipyge condition may be caused by an increase in the fractional rate of protein synthesis.

published proceedings

  • J Anim Sci

author list (cited authors)

  • Lorenzen, C. L., Koohmaraie, M., Shackelford, S. D., Jahoor, F., Freetly, H. C., Wheeler, T. L., Savell, J. W., & Fiorotto, M. L.

citation count

  • 45

complete list of authors

  • Lorenzen, CL||Koohmaraie, M||Shackelford, SD||Jahoor, F||Freetly, HC||Wheeler, TL||Savell, JW||Fiorotto, ML

publication date

  • January 2000