Isolation and mapping of polymorphic microsatellites in cattle Academic Article uri icon

abstract

  • A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC-dA)n.(dG-dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR-analysis of a panel of somatic hybrid lines.

author list (cited authors)

  • Steffen, P., Eggen, A., Stranzinger, G., Fries, R., Dietz, A. B., & Womack, J. E.

citation count

  • 120

complete list of authors

  • Steffen, P||Eggen, A||Dietz, AB||Womack, JE||Stranzinger, G||Fries, R

publication date

  • April 1993

publisher