Isolation and mapping of polymorphic microsatellites in cattle. Academic Article

abstract

• A partial plasmid library with bovine genomic inserts of about 500 basepairs was screened with a (dC-dA)n.(dG-dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with three alleles and two with two alleles. Six of these microsatellites were mapped by PCR-analysis of a panel of somatic hybrid lines.

authors

• Womack, James

published proceedings

• Anim Genet

author list (cited authors)

• Steffen, P., Eggen, A., Dietz, A. B., Womack, J. E., Stranzinger, G., & Fries, R.

citation count

• 124

complete list of authors

• Steffen, P||Eggen, A||Dietz, AB||Womack, JE||Stranzinger, G||Fries, R

publication date

• April 1993

publisher

• Wiley  Publisher

published in

• Animal Genetics  Journal

keywords

• Alleles
• Animals
• Base Sequence
• Cattle
• Chromosome Mapping
• DNA, Satellite
• Electrophoresis, Polyacrylamide Gel
• Gene Library
• Genetic Markers
• Molecular Sequence Data
• Polymerase Chain Reaction
• Polymorphism, Genetic

PubMed Central ID

• 8328693

Digital Object Identifier (DOI)

• 10.1111/j.1365-2052.1993.tb00252.x

start page

• 121

end page

• 124

volume

• 24

issue

• 2

URL

• http://dx.doi.org/10.1111/j.1365-2052.1993.tb00252.x