Isolation and mapping of a single-locus minisatellite sequence marker to 1q36-qter and synteny group 10 in cattle and to 1q36-qter in sheep. Academic Article

abstract

• A minisatellite sequence (RD1613) was isolated from a bovine cosmid genomic library and its chromosomal location determined in cattle and sheep. In cattle, somatic cell hybrid panel analysis assigned RD1613 to the syntenic group U10 with a concordancy of 100%. In situ hybridization placed RD1613 onto bovine chromosome 1 in the region of bands q36-qter. This is the first in situ localization to chromosome 1 in cattle and allows the provisional assignment of syntenic group U10 to this chromosome. It was also found that RD1613 hybridized strongly to sheep genomic DNA. In situ hybridization localized RD1613 to sheep chromosome 1q36-qter, which is consistent with homology between cattle chromosome 1 and sheep chromosome 1q. The RD1613 probe detects a polymorphic single locus marker (designated D1S1) in both cattle and sheep and will be very useful in linkage studies.

authors

• Womack, James

published proceedings

• Genomics

author list (cited authors)

• Drinkwater, R. D., Johnson, S. E., Barendse, W., Harrison, B., Womack, J. E., & Hetzel, D. J.

citation count

• 6

complete list of authors

• Drinkwater, RD||Johnson, SE||Barendse, W||Harrison, B||Womack, JE||Hetzel, DJ

publication date

• January 1994

publisher

• Elsevier  Publisher

published in

• Genomics  Journal

keywords

• Animals
• Cattle
• Chromosome Banding
• Chromosome Mapping
• Cosmids
• Gene Library
• Genetic Markers
• In Situ Hybridization
• Repetitive Sequences, Nucleic Acid
• Sheep
• Species Specificity

PubMed Central ID

• 8188216

Digital Object Identifier (DOI)

• 10.1006/geno.1994.1025

start page

• 149

end page

• 151

volume

• 19

issue

• 1

URL

• http://dx.doi.org/10.1006/geno.1994.1025