Identification of an autosome to X chromosome translocation in the domestic cow.
Academic Article
Overview
Research
Identity
Additional Document Info
Other
View All
Overview
abstract
Chromosomes were harvested from two fibroblast lines derived from a phenotypically normal cow and her albino daughter, both known to be heterozygous for an X/autosome translocation. QFH-banding after early BrdU incorporation identified the translocated autosomal material as chromosome 23 bands 13-25, and revealed that the centromeric portion of the translocated chromosome 23 had been retained. Fluorescent in situ hybridization of a BoLA Class I cDNA probe to the normal chromosome 23 and to the translocated autosomal material confirmed the identity of the translocation, and allowed a more precise sublocalization of the MHC in cattle than that previously reported.