Syntenic mapping and chromosomal localization of bovine alpha and beta interferon genes. Academic Article

abstract

• The previous assignment of bovine alpha-(IFNA) and beta-(IFNB) interferon gene families to syntenic group U18 was confirmed with additional cDNA probes and a bovine-rodent hybrid somatic cell panel representing all 29 bovine autosomal syntenic groups. Fluorescent in situ hybridization (FISH) localized these genes to bovine Chromosome (Chr) 8 band 15 and demonstrates that with biotinylated plasmids, as few as five tandemly arrayed sequences can be detected by conventional fluorescent microscopy. This technique can be applied to physical mapping of other multicopy genes in domestic animals.

authors

• Womack, James

published proceedings

• Mamm Genome

author list (cited authors)

• Ryan, A. M., Gallagher, D. S., & Womack, J. E.

citation count

• 14

complete list of authors

• Ryan, AM||Gallagher, DS||Womack, JE

publication date

• October 1992

publisher

• Springer Nature  Publisher

keywords

• Animals
• Cattle
• Chromosome Mapping
• Hybrid Cells
• In Situ Hybridization, Fluorescence
• Interferon-alpha
• Interferon-beta

Digital Object Identifier (DOI)

• 10.1007/BF00350624

start page

• 575

end page

• 578

volume

• 3

issue

• 10

URL

• http://dx.doi.org/10.1007/bf00350624