Amino acid sequence of porcine heart fumarase. Academic Article

abstract

• The complete amino acid sequence of porcine heart fumarase (EC 4.2.1.2) has been determined from peptides produced by cyanogen bromide, endoproteinase Arg-C, S. aureus V8 protease, and trypsin. The enzyme is a tetramer of identical subunits with Mr = 50,015 and composed of 466 amino acid residues. Porcine heart fumarase displays 96% identity to human liver fumarase. Prediction of the secondary structural elements of porcine fumarase indicate that the enzyme contains a large amount of alpha helix with very little beta structure.

authors

• Sacchettini, James

published proceedings

• Biochem Biophys Res Commun

author list (cited authors)

• Sacchettini, J. C., Frazier, M. W., Chiara, D. C., Banaszak, L. J., & Grant, G. A.

citation count

• 18

complete list of authors

• Sacchettini, JC||Frazier, MW||Chiara, DC||Banaszak, LJ||Grant, GA

publication date

• May 1988

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Amino Acid Sequence
• Animals
• Fumarate Hydratase
• Molecular Sequence Data
• Myocardium
• Peptide Mapping
• Protein Conformation
• Swine

PubMed Central ID

• 3377794

Digital Object Identifier (DOI)

• 10.1016/s0006-291x(88)81243-9

start page

• 435

end page

• 440

volume

• 153

issue

• 1

URL

• http://dx.doi.org/10.1016/s0006-291x(88)81243-9