Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models.

abstract

• The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We describe steps for decalcifying bone, using solvents to remove color and lipids, and imaging tissues in 3D using confocal and light sheet microscopy. We then detail a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software. For complete details on the use and execution of this protocol, please refer to (Moatti etal., 2022).1.

authors

• Ligler, Frances

published proceedings

• STAR Protoc

author list (cited authors)

• Moatti, A., Cai, Y., Li, C., Popowski, K. D., Cheng, K. e., Ligler, F. S., & Greenbaum, A.

complete list of authors

• Moatti, Adele||Cai, Yuheng||Li, Chen||Popowski, Kristen D||Cheng, Ke||Ligler, Frances S||Greenbaum, Alon

publisher

• Elsevier  Publisher

published in

• STAR Protocols  Journal

keywords

• Biotechnology And Bioengineering
• Microscopy

PubMed Central ID

• 37060559

Digital Object Identifier (DOI)

• 10.1016/j.xpro.2023.102220

start page

• 102220

end page

• 102220

volume

• 4

issue

• 2