Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models. Academic Article uri icon

abstract

  • The inner ear of humans and large animals is embedded in a thick and dense bone that makes dissection challenging. Here, we present a protocol that enables three-dimensional (3D) characterization of intact inner ears from large-animal models. We describe steps for decalcifying bone, using solvents to remove color and lipids, and imaging tissues in 3D using confocal and light sheet microscopy. We then detail a pipeline to count hair cells in antibody-stained and 3D imaged cochleae using open-source software. For complete details on the use and execution of this protocol, please refer to (Moatti etal., 2022).1.

published proceedings

  • STAR Protoc

altmetric score

  • 0.25

author list (cited authors)

  • Moatti, A., Cai, Y., Li, C., Popowski, K. D., Cheng, K. e., Ligler, F. S., & Greenbaum, A.

citation count

  • 0

complete list of authors

  • Moatti, Adele||Cai, Yuheng||Li, Chen||Popowski, Kristen D||Cheng, Ke||Ligler, Frances S||Greenbaum, Alon

publication date

  • April 2023