CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator Academic Article uri icon

abstract

  • Copper is an essential element that becomes highly cytotoxic when concentrations exceed the capacity of cells to sequester the ion. Here, we identify a new copper-specific repressor (CsoR) of a copper-sensitive operon (cso) in Mycobacterium tuberculosis (Mtb) that is representative of a large, previously uncharacterized family of proteins (DUF156). Electronic and X-ray absorption spectroscopies reveal that CsoR binds a single-monomer mole equivalent of Cu(I) to form a trigonally coordinated (S(2)N) Cu(I) complex. The 2.6-A crystal structure of copper-loaded CsoR shows a homodimeric antiparallel four-helix bundle architecture that represents a novel DNA-binding fold. The Cu(I) is coordinated by Cys36, Cys65' and His61' in a subunit bridging site. Cu(I) binding negatively regulates the binding of CsoR to a DNA fragment encompassing the operator-promoter region of the Mtb cso operon; this results in derepression of the operon in Mtb and the heterologous host Mycobacterium smegmatis. Substitution of Cys36 or His61 with alanine abolishes Cu(I)- and CsoR-dependent regulation in vivo and in vitro. Potential roles of CsoR in Mtb pathogenesis are discussed.

altmetric score

  • 4.5

author list (cited authors)

  • Liu, T., Ramesh, A., Ma, Z., Ward, S. K., Zhang, L., George, G. N., ... Giedroc, D. P.

citation count

  • 247

complete list of authors

  • Liu, Tong||Ramesh, Arati||Ma, Zhen||Ward, Sarah K||Zhang, Limei||George, Graham N||Talaat, Adel M||Sacchettini, James C||Giedroc, David P

publication date

  • December 2006