A whole-genome radiation hybrid panel for goat Academic Article uri icon

abstract

  • A 5000. rad goat-hamster panel of 121 whole-genome radiation hybrids was generated and preliminarily characterized. A normal diploid fibroblast culture from a male Boer goat was fused with a recipient thymidine kinase-deficient hamster cell line. The generated 121 radiation hybrids were grown and produced an average of 8.4. mg of DNA per radiation hybrid. A SINE-PCR test showed that almost all radiation hybrids retained goat DNA. The retention frequencies of the 121 hybrids were preliminarily estimated using a collection of 42 unlinked molecular markers. An optimized panel of therein 90 radiation hybrids (CHRH5000) with an average retention frequency of 34.2% was screened on the basis of interspersed repetitive DNA content and chromosome retention frequency. The development of this WG-RH panel will provide a fundamental tool for advanced goat genome mapping studies and for mammalian comparative mapping. 2012 Elsevier B.V.

published proceedings

  • Small Ruminant Research

author list (cited authors)

  • Du, X. Y., Womack, J. E., Owens, K. E., Elliott, J. S., Sayre, B., Bottcher, P. J., ... Malek, M.

citation count

  • 10

complete list of authors

  • Du, XY||Womack, JE||Owens, KE||Elliott, JS||Sayre, B||Bottcher, PJ||Milan, D||Garcia Podesta, M||Zhao, SH||Malek, M

publication date

  • June 2012