A whole-genome radiation hybrid panel for goat
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A 5000. rad goat-hamster panel of 121 whole-genome radiation hybrids was generated and preliminarily characterized. A normal diploid fibroblast culture from a male Boer goat was fused with a recipient thymidine kinase-deficient hamster cell line. The generated 121 radiation hybrids were grown and produced an average of 8.4. mg of DNA per radiation hybrid. A SINE-PCR test showed that almost all radiation hybrids retained goat DNA. The retention frequencies of the 121 hybrids were preliminarily estimated using a collection of 42 unlinked molecular markers. An optimized panel of therein 90 radiation hybrids (CHRH5000) with an average retention frequency of 34.2% was screened on the basis of interspersed repetitive DNA content and chromosome retention frequency. The development of this WG-RH panel will provide a fundamental tool for advanced goat genome mapping studies and for mammalian comparative mapping. © 2012 Elsevier B.V.
author list (cited authors)
Du, X. Y., Womack, J. E., Owens, K. E., Elliott, J. S., Sayre, B., Bottcher, P. J., ... Malek, M.
complete list of authors
Du, XY||Womack, JE||Owens, KE||Elliott, JS||Sayre, B||Bottcher, PJ||Milan, D||Garcia Podesta, M||Zhao, SH||Malek, M