A high-resolution radiation hybrid map of sheep chromosome X and comparison with human and cattle.
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A radiation hybrid (RH) map of sheep X chromosome (Ovisaries; OARX) containing 146 physically anchored loci was generated in this study, providing information for comparative X chromosome analysis between the maps of sheep, human, and cattle. Primers typed on the USUoRH5000 ovine whole-genome radiation hybrid panel were designed from sequences predicted to be on the ovine X chromosome, based on comparative mapping within the virtual sheep genome browser (v1.2). The resulting RH map for the ovine X chromosome consists of 4 linkage groups composed of 76 BAC end sequences (BES), 28 gene loci that were confirmed within ovine BAC clones in the CHORI-243 ovine BAC library, 28 additional gene loci from the ovine comparative map and 14 polymorphic sequence tagged sites (STS) from the OARX linkage map. This first-generation RH map of OARX contributes to the expansion of a comprehensive ovine genome map for sheep and provides evidence of rearrangements in loci order compared to the human and cattle orders.
author list (cited authors)
Goldammer, T., Brunner, R. M., Rebl, A., Wu, C. H., Nomura, K., Hadfield, T., ... Cockett, N. E.
complete list of authors
Goldammer, T||Brunner, RM||Rebl, A||Wu, CH||Nomura, K||Hadfield, T||Gill, C||Dalrymple, BP||Womack, JE||Cockett, NE