Detection of polymorphisms in bovine toll-like receptors 3, 7, 8, and 9. Academic Article

abstract

• The toll-like receptors (TLRs) detect molecular signatures of invaders known as pathogen-associated molecular patterns (PAMPs). Ten members of the TLR family have been identified in cattle to date and 4 of these recognize PAMPs specific to viruses (TLRs 3, 7, 8, 9). The objective of this work was to detect polymorphisms in the genomic sequences of bovine TLRs 3, 7, 8, and 9. To achieve this objective, a panel of nine breeds representing Bos taurus and Bos indicus was assembled for sequencing and comparison with the Bovine Genome Project sequence. Comparative sequence analysis revealed a total of 139 polymorphisms, which include single-nucleotide polymorphisms and insertion-deletion polymorphisms. Of the 139 polymorphisms, 88% (N=123) are novel. In addition, the protein domain architecture of these four TLRs was examined between human, mouse, cow, and dog, which revealed several regions of conservation in the TLR variable leucine-rich-repeat patterning.

authors

• Womack, James

published proceedings

• Genomics

author list (cited authors)

• Cargill, E. J., & Womack, J. E.

citation count

• 47

complete list of authors

• Cargill, EJ||Womack, JE

publication date

• June 2007

publisher

• Elsevier  Publisher

published in

• Genomics  Journal

keywords

• Animals
• Base Sequence
• Cattle
• Conserved Sequence
• DNA Primers
• Immunity, Innate
• Molecular Sequence Data
• Polymorphism, Genetic
• Polymorphism, Single Nucleotide
• Protein Structure, Tertiary
• Species Specificity
• Toll-Like Receptor 3
• Toll-Like Receptor 7
• Toll-Like Receptor 8
• Toll-Like Receptor 9
• Toll-Like Receptors

PubMed Central ID

• 17442537

Digital Object Identifier (DOI)

• 10.1016/j.ygeno.2007.02.008

start page

• 745

end page

• 755

volume

• 89

issue

• 6

URL

• http://dx.doi.org/10.1016/j.ygeno.2007.02.008