Visualization of pronuclei in living bovine zygotes. Academic Article uri icon


  • Bovine embryos were surgically collected from the oviducts of superovulated crossbred heifers 48 h postonset of estrus. The one-celled ova were treated with 4'-6'-diamidino-2-phenylindole (DAPI) and observed under ultraviolet light by fluorescence microscopy. Both male and female pronuclei were visualized, identified and subjected to micromanipulation.

published proceedings

  • Biol Reprod

author list (cited authors)

  • Minhas, B. S., Capehart, J. S., Bowen, M. J., Womack, J. E., McCrady, J. D., Harms, P. G., Wagner, T. E., & Kraemer, D. C.

citation count

  • 23

complete list of authors

  • Minhas, BS||Capehart, JS||Bowen, MJ||Womack, JE||McCrady, JD||Harms, PG||Wagner, TE||Kraemer, DC

publication date

  • April 1984