Chain elongation of eicosapentaenoic acid in the macrophage. Academic Article

abstract

• In order to elucidate the metabolic fate of eicosapentaenoic acid (20:5 (n-3], a major n-3 fatty acid constituent of fish oil, resident and casein-elicited mouse peritoneal macrophages were incubated with [3H]20:5 (n-3). Comparative experiments with arachidonic acid (20:4 (n-6] were also conducted. After 4, 8 and 18 h incubation, [3H]20:5 (n-3) was extensively elongated into [3H]22:5(n-3) while [3H]20:4(n-6) was only moderately elongated into [3H]22:4(n-6) in both resident and elicited macrophages. No measurable conversion of [3H]22:5(n-3) into [3H]22:6(n-3) (delta 4 desaturation) could be demonstrated. These data demonstrate that the highly active chain elongation of 20:5(n-3) by macrophage elongase, as well as the lack of detectable delta 4 desaturase activity, are responsible for the accumulation of 22:5(n-3) in this cell.

authors

• Chapkin, Robert

published proceedings

• Biochim Biophys Acta

author list (cited authors)

• Chapkin, R. S., & Miller, C. C.

citation count

• 24

complete list of authors

• Chapkin, RS||Miller, CC

publication date

• February 1990

publisher

• Elsevier  Publisher

published in

• Biochimica et Biophysica Acta: international journal of biochemistry and biophysics  Journal

keywords

• Animals
• Chromatography, High Pressure Liquid
• Chromatography, Thin Layer
• Eicosapentaenoic Acid
• Fatty Acids, Unsaturated
• Female
• Macrophages
• Mice
• Mice, Inbred C57BL
• Peritoneal Cavity

PubMed Central ID

• 2302426

Digital Object Identifier (DOI)

• 10.1016/0005-2760(90)90019-t

start page

• 265

end page

• 267

volume

• 1042

issue

• 2

URL

• http://dx.doi.org/10.1016/0005-2760(90)90019-t