Parallel radiation hybrid mapping: a powerful tool for high-resolution genomic comparison. Academic Article

abstract

• Comparative gene mapping in mammals typically involves identification of segments of conserved synteny in diverse genomes. The development of maps that permit comparison of gene order within conserved synteny has not advanced beyond the mouse map that takes advantage of linkage analysis in interspecific backcrosses. Radiation hybrid (RH) mapping provides a powerful tool for determining order of genes in genomes for which gene-based linkage mapping is impractical. Comparative RH mapping of 24 orthologous genes in this study revealed internal structural rearrangements between human chromosome 17 (HSA17) and bovine chromosome 19 (BTA19), two chromosomes known previously to be conserved completely and exclusively at level of synteny. Only six of the 24 genes had been previously ordered on the human G3 RH map. The use of the G3 panel to map the other 18, however, produced parallel RH maps for comparison of gene order at a resolution of <5 Mb on the bovine linkage map and from 1 to 3 Mb in the human physical map.

authors

• Womack, James

published proceedings

• Genome Res

author list (cited authors)

• Yang, Y. P., & Womack, J. E.

citation count

• 43

complete list of authors

• Yang, YP||Womack, JE

publication date

• July 1998

publisher

• Cold Spring Harbor Laboratory  Publisher

published in

• Genome Research  Journal

keywords

• Animals
• Cattle
• Chromosome Mapping
• Chromosomes, Human, Pair 17
• Genetic Markers
• Genomic Library
• Humans
• Hybrid Cells
• Translocation, Genetic

PubMed Central ID

• 9685320

Digital Object Identifier (DOI)

• 10.1101/gr.8.7.731

start page

• 731

end page

• 736

volume

• 8

issue

• 7

URL

• http://dx.doi.org/10.1101/gr.8.7.731