Physical mapping of inhibin beta-A in domestic cattle. Academic Article

abstract

• The gene for the beta-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.

authors

• Womack, James

published proceedings

• Mamm Genome

author list (cited authors)

• Neibergs, H. L., Gallagher, D. S., Georges, M., Sargeant, L. S., Dietz, A. B., & Womack, J. E.

citation count

• 20

publication date

• June 1993

publisher

• Springer Nature  Publisher

keywords

• Animals
• Base Sequence
• CHO Cells
• Cattle
• Chromosome Mapping
• Cricetinae
• DNA, Single-Stranded
• Hybrid Cells
• In Situ Hybridization, Fluorescence
• Inhibins
• Molecular Sequence Data
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Polymorphism, Restriction Fragment Length

Digital Object Identifier (DOI)

• 10.1007/BF00357092

start page

• 328

end page

• 332

volume

• 4

issue

• 6

URL

• http://dx.doi.org/10.1007/bf00357092