A buoyancy-driven compact thermocycler for rapid PCR. Academic Article

abstract

• The authors have designed a novel convective flow-based thermocycling system capable of performing high-speed DNA amplification via the polymerase chain reaction in a simplified and inexpensive format. Successful amplification of a 191 bp influenza-A target is demonstrated within 25 minutes using a 10 muL reaction volume with no modification to standard laboratory protocols. The system is simple to assemble and can be readily integrated with existing laboratory instrumentation for automated operation.

authors

• Ugaz, Victor

published proceedings

• Clin Lab Med

author list (cited authors)

• Agrawal, N., & Ugaz, V. M.

citation count

• 18

complete list of authors

• Agrawal, Nitin||Ugaz, Victor M

publication date

• March 2007

publisher

• Elsevier  Publisher

published in

• Clinics in Laboratory Medicine  Journal

keywords

• Automation
• DNA
• Hot Temperature
• Influenza A Virus
• Polymerase Chain Reaction
• Time Factors

PubMed Central ID

• 17416315

Digital Object Identifier (DOI)

• 10.1016/j.cll.2007.01.004

start page

• 215

end page

• 223

volume

• 27

issue

• 1

URL

• http://dx.doi.org/10.1016/j.cll.2007.01.004