Microscale chaotic advection enables robust convective DNA replication. Academic Article

abstract

• The ability of chaotic advection under microscale confinement to direct chemical processes along accelerated kinetic pathways has been recognized for some time. However, practical applications have been slow to emerge because optimal results are often counterintuitively achieved in flows that appear to possess undesirably high disorder. Here we present a 3D time-resolved analysis of polymerase chain reaction (PCR)-mediated DNA replication across a broad ensemble of geometric states. The resulting parametric map reveals an unexpectedly wide operating regime where reaction rates remain constant over 2 orders of magnitude of the Rayleigh number, encompassing virtually any realistic PCR condition (temperature, volume, gravitational alignment), a level of robustness previously thought unattainable in the convective format.

authors

• Hassan, Yassin
• Ugaz, Victor

published proceedings

• Anal Chem

altmetric score

• 6.5

author list (cited authors)

• Priye, A., Hassan, Y. A., & Ugaz, V. M.

citation count

• 15

complete list of authors

• Priye, Aashish||Hassan, Yassin A||Ugaz, Victor M

publication date

• November 2013

publisher

• American Chemical Society (ACS)  Publisher

published in

• Analytical Chemistry  Journal

keywords

• DNA Replication
• Kinetics
• Nonlinear Dynamics

PubMed Central ID

• 24083802

Digital Object Identifier (DOI)

• 10.1021/ac402611s

start page

• 10536

end page

• 10541

volume

• 85

issue

• 21

URL

• http://dx.doi.org/10.1021/ac402611s