Description of bovine major histocompatibility complex class IIa haplotypes using parthenogenetic embryo-derived cells. Academic Article uri icon

abstract

  • In this study, we report an approach to characterize individual BoLA haplotypes using cells from parthenogenetic bovine embryos derived from slaughterhouse ovaries. Eight of the 15 parthenogenetic embryos so obtained had not undergone meiotic recombination on the BoLA region and were suitable to describe BoLA haplotypes. Detailed analysis of the BoLA class IIa region identified seven different class IIa haplotypes, including six not previously described and two new alleles of BoLA-DQA and one BoLA-DQB. Our method provided reliable sources of homozygous DNA to describe BoLA haplotypes.

published proceedings

  • Anim Genet

author list (cited authors)

  • S, A., Leal, V., Schneider, M., Ohashi, O. M., Santos, E., Riggs, P. K., ... Sena, L

citation count

  • 0

complete list of authors

  • Sá, ALA||Leal, VSG||Schneider, MPC||Ohashi, OM||Santos, EJM||Riggs, PK||Kochan, KJ||Gill, CA||Downey, ED||Womack, JE||Skow, LC||Miranda, MS||Sena, L

publication date

  • June 2015

publisher