Gene discovery and comparative analysis of X-degenerate genes from the domestic cat Y chromosome.
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Mammalian sex chromosomes are the remnants of an ancient autosomal pair present in the ancestral mammalian karyotype. As a consequence of random decay and chromosome rearrangements over evolutionary time, Y chromosome gene repertoires differ between eutherian lineages. To investigate the gene repertoire and transcriptional analysis of the domestic cat Y chromosome, and their potential roles in spermatogenesis, we obtained full-length cDNA sequences for all known Y genes and their X chromosome gametologues and used those sequences to create a BAC-based physical map of the X-degenerate region. Our results indicate the domestic cat Y chromosome has retained most X-degenerate genes that were present on the ancestral eutherian Y chromosome. Transcriptional analysis revealed that most feline X-degenerate genes have retained housekeeping functions shared by their X chromosome partners and have not been specialized for testis-specific functions. Physical mapping data indicate that the cat SRY gene is present as multiple functional copies and that very little of the felid Y chromosome may be single copy. X-Y gene divergence time estimates obtained using Bayesian methods confirm an early origin of Stratum 1 genes prior to the origin of therian mammals. We observed no statistical difference in the ages of Stratum 2 and Stratum 3 gene pairs, suggesting that eutherian and marsupial Stratum 2 genes may have been independently retained in each lineage.
author list (cited authors)
Pearks Wilkerson, A. J., Raudsepp, T., Graves, T., Albracht, D., Warren, W., Chowdhary, B. P., Skow, L. C., & Murphy, W. J.
complete list of authors
Pearks Wilkerson, Alison J||Raudsepp, Terje||Graves, Tina||Albracht, Derek||Warren, Wesley||Chowdhary, Bhanu P||Skow, Loren C||Murphy, William J