Advances in nonlinear optical microscopy for visualizing dynamic tissue properties in culture. Academic Article

abstract

• Optical microscopy encompasses high-resolution imaging techniques that can be used to non-destructively investigate and characterize living biological systems and engineered tissue constructs in culture. In particular, nonlinear optical microscopy (NLOM) is well suited for the visualization and quantification of processes involved in cell-extracellular matrix interactions in vivo. Current NLOM technology enables concomitant molecular imaging and visualization of microstructural organization that could provide a direct link between signal transduction and biological effect at microscopic length scales that culminate into tissue macroscopic properties and function. This review highlights the fundamentals of nonlinear optical interactions between light and tissue and presents a direction for future technology development to better complement quantitative, high-throughput assays of the modern life sciences.

authors

• Gibbs, Holly

published proceedings

• Tissue Eng Part B Rev

author list (cited authors)

• Yeh, A. T., Gibbs, H., Hu, J., & Larson, A. M.

citation count

• 15

complete list of authors

• Yeh, Alvin T||Gibbs, Holly||Hu, Jin-Jia||Larson, Adam M

publication date

• March 2008

publisher

• Mary Ann Liebert  Publisher

published in

• Tissue Engineering Part B: Reviews  Journal

keywords

• Cell Survival
• Collagen
• Culture Media
• Culture Techniques
• Extracellular Matrix
• Fibroblasts
• Image Processing, Computer-Assisted
• Imaging, Three-Dimensional
• Light
• Microbiological Techniques
• Microscopy
• Models, Statistical
• Optics And Photonics
• Photons
• Signal Transduction

PubMed Central ID

• 18454638

Digital Object Identifier (DOI)

• 10.1089/teb.2007.0284

start page

• 119

end page

• 131

volume

• 14

issue

• 1

URL

• http://dx.doi.org/10.1089/teb.2007.0284