Ex vivo characterization of sub-10-fs pulses. Academic Article uri icon


  • An all-mirror dispersion-compensation setup is used to correct for quadratic and cubic phase distortions induced within a custom nonlinear optical microscope. Mouse tail tendon is used to characterize sub-10-fs pulses by interferometric autocorrelation. This is an ideal method for characterizing dispersion from the optical system, immersion medium, and wet biological sample. The generation of very short autocorrelations demonstrates the ability to compensate for phase distortions within the imaging system and efficient second-harmonic upconversion of the ultrashort pulse spectrum within collagen. Compensated autocorrelation traces are presented for biologically relevant objective lenses.

author list (cited authors)

  • Larson, A. M., & Yeh, A. T.

citation count

  • 44

publication date

  • June 2006