Safety and immunogenicity of a glycoprotein E gene-deleted bovine herpesvirus 1 strain as a candidate vaccine strain Academic Article uri icon


  • ABSTRACT: A glycoprotein E-deleted Brazilian bovine herpesvirus 1 (BoHV-1gE) was tested regarding to safety and immunogenicity. Intramuscular inoculation of young calves with a high virus dose did not result in clinical signs or virus shedding during acute infection or after dexamethasone administration. Calves vaccinated once IM (group I) or subcutaneously (group II) with live BoHV-1gE or twice with inactivated virus plus aluminum hydroxide (group IV) or Montanide (group V) developed VN titers of 2 to 8 (GMT:2); 2 to 4 (GMT:1.65); 2 to 16 (GMT:2.45) and 2 to 128 (GMT:3.9), respectively. All BoHV-1gE vaccinated calves remained negative in an anti-gE ELISA. Lastly, six young calves vaccinated with live BoHV-1gE and subsequently challenged with a virulent BoHV-1 strain shed less virus and developed only mild and transient nasal signs comparing to unvaccinated calves. Thus, the recombinant BoHV-1gE is safe and immunogenic for calves and allows for serological differentiation by a gE-ELISA test.

published proceedings


author list (cited authors)

  • Weiss, M., Anziliero, D., Martins, M., Weiblen, R., & Flores, E. F.

citation count

  • 2

complete list of authors

  • Weiss, Marcelo||Anziliero, Deniz||Martins, Mathias||Weiblen, Rudi||Flores, Eduardo Furtado

publication date

  • November 2016