Applications of CRISPR/Cas13-Based RNA Editing in Plants. Academic Article

abstract

• The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is widely used as a genome-editing tool in various organisms, including plants, to elucidate the fundamental understanding of gene function, disease diagnostics, and crop improvement. Among the CRISPR/Cas systems, Cas9 is one of the widely used nucleases for DNA modifications, but manipulation of RNA at the post-transcriptional level is limited. The recently identified type VI CRISPR/Cas systems provide a platform for precise RNA manipulation without permanent changes to the genome. Several studies reported efficient application of Cas13 in RNA studies, such as viral interference, RNA knockdown, and RNA detection in various organisms. Cas13 was also used to produce virus resistance in plants, as most plant viruses are RNA viruses. However, the application of CRISPR/Cas13 to studies of plant RNA biology is still in its infancy. This review discusses the current and prospective applications of CRISPR/Cas13-based RNA editing technologies in plants.

authors

• Mandadi, Kranthi

published proceedings

• Cells

altmetric score

• 0.25

author list (cited authors)

• Kavuri, N. R., Ramasamy, M., Qi, Y., & Mandadi, K.

citation count

• 4

complete list of authors

• Kavuri, Naga Rajitha||Ramasamy, Manikandan||Qi, Yiping||Mandadi, Kranthi

publication date

• August 2022

publisher

• MDPI  Publisher

published in

• Cells  Journal

keywords

• CRISPR-Cas Systems
• Crispr/cas13
• Gene Editing
• Plants
• RNA
• RNA Interference
• Rna Editing
• Rna Targeting
• Type Vi Crispr Systems

PubMed Central ID

• 36078073

Digital Object Identifier (DOI)

• 10.3390/cells11172665

start page

• 2665

end page

• 2665

volume

• 11

issue

• 17

URL

• http://dx.doi.org/10.3390/cells11172665