Agrobacterium-mediated DNA transfer in sugar pine. Academic Article

abstract

• DNA transfer using Agrobacterium tumefaciens has been demonstrated in sugar pine, Pinus lambertiana Dougl. Shoots derived from cytokinin-treated cotyledons formed galls after inoculation with A. tumefaciens strains containing the plasmid pTiBo542. A selectable marker, neomycin phosphotransferase II, conferring resistance to kanamycin, was transferred into sugar pine using a binary armed vector system. Callus proliferated from the galls grew without hormones and in some cases, kanamycin-resistant callus could be cultured. Southern blots provided evidence of physical transfer of T-DNA and the nptII gene. Expression of the nptII gene under control of the nos promoter was demonstrated by neomycin phosphotransferase assays. Several aspects of DNA transfer were similar to those previously observed in angiosperms transformed by A. tumefaciens. This is the first evidence for DNA transfer by Agrobacterium in this species and the first physical evidence for transfer in any pine. These results bring us closer to genetic engineering in this commercially important genus of forest trees.

authors

• Loopstra, Carol

published proceedings

• Plant Mol Biol

altmetric score

• 3

author list (cited authors)

• Loopstra, C. A., Stomp, A. M., & Sederoff, R. R.

citation count

• 49

complete list of authors

• Loopstra, CA||Stomp, AM||Sederoff, RR

publication date

• July 1990

publisher

• Springer Nature  Publisher

keywords

• DNA
• DNA, Bacterial
• Gene Expression
• Genetic Engineering
• Genetic Markers
• Genetic Vectors
• Kanamycin Kinase
• Phosphotransferases
• Plants
• Plasmids
• Restriction Mapping
• Rhizobium
• Transfection

Digital Object Identifier (DOI)

• 10.1007/BF00017719

start page

• 1

end page

• 9

volume

• 15

issue

• 1

URL

• http://dx.doi.org/10.1007/bf00017719