Simple, filter-based PCR detection of Thelohania solenopsae (Microspora) in fire ants (Solenopsis invicta).

abstract

• Thelohania solenopsae is a microsporidian parasite that may serve as a biological control agent for the red imported fire ant, Solenopsis invicta. A rapid, filter-based PCR amplification method detecting a portion of the small-subunit ribosomal RNA gene was developed to facilitate field studies detecting the parasite in fire ants. Processing ant homogenates with a commercially available membrane-based system, FTA Classic Card technology, compared favorably with traditional DNA extraction and PCR amplification methods. As few as 100 spores were detected. The FTA membrane system is a simple, extraction-free method for detecting T. solenopsae in fire ants, and allows for easy archival storage of DNA samples.

authors

• Snowden, Karen

published proceedings

• J Eukaryot Microbiol

author list (cited authors)

• Snowden, K. F., Logan, K. S., & Vinson, S. B.

citation count

• 15

complete list of authors

• Snowden, Karen F||Logan, Kathleen S||Vinson, S Bradleigh

publication date

• November 2002

publisher

• Wiley  Publisher

published in

• Journal of Eukaryotic Microbiology  Journal

keywords

• Animals
• Ants
• DNA, Protozoan
• Filtration
• Microsporidia
• Polymerase Chain Reaction
• Sensitivity And Specificity
• Time Factors

PubMed Central ID

• 12503678

Digital Object Identifier (DOI)

• 10.1111/j.1550-7408.2002.tb00226.x

start page

• 447

end page

• 448

volume

• 49

issue

• 6

URL

• http://dx.doi.org/10.1111/j.1550-7408.2002.tb00226.x