Isolation, characterization, and molecular identification of bacteria from the red imported fire ant (Solenopsis invicta) midgut. Academic Article

abstract

• Bacteria were isolated and cultured from the red imported fire ant (Solenopsis invicta) midgut. The small-subunit ribosomal RNA gene, (16s rRNA gene, approximately 1500 bp) was amplified from bacterial genomic DNA using the polymerase chain reaction and consensus sequence primers. Restriction fragment length polymorphism analysis revealed 10 unique profiles, indicating that at least 10 different bacteria are present in red imported fire ant midguts. The 16s rRNA gene sequence was determined for these isolates and queried against the NCBI genetic database. The results identified all isolates to at least the genus level. Antibiotic resistance profiles and biochemical activities were also determined for these species. This work provides the basis for a wider characterization of bacterial distributions in fire ant colonies and provides strains suitable for genetic manipulation to develop novel methods of fire ant control.

authors

• Coates, Craig

published proceedings

• J Invertebr Pathol

author list (cited authors)

• Li, H., Medina, F., Vinson, S. B., & Coates, C. J.

citation count

• 48

complete list of authors

• Li, Haiwen||Medina, Freder||Vinson, S Bradleigh||Coates, Craig J

publication date

• January 2005

publisher

• Elsevier  Publisher

published in

• Journal of Invertebrate Pathology  Journal

keywords

• Animals
• Ants
• Bacteria
• DNA, Bacterial
• Digestive System
• Larva
• Pest Control, Biological
• Phenotype
• Polymorphism, Restriction Fragment Length
• RNA, Bacterial
• RNA, Ribosomal, 16S

Digital Object Identifier (DOI)

• 10.1016/j.jip.2005.05.008

start page

• 203

end page

• 209

volume

• 89

issue

• 3

URL

• http://dx.doi.org/10.1016/j.jip.2005.05.008