Electrokinetically actuated protein crystallization Conference Paper uri icon


  • Currently, x-ray crystallography is the primary experimental tool employed to understand how proteins fold and assemble into well-defined structures. These diffraction methods critically depend the ability to produce high-purity crystal samples, but conventional crystallization processes are inherently slow and challenging to control. Here we explore a fundamentally different electrokinetically-actuated approach to overcome these limitations. Instead of the conventional method involving increasing protein concentration throughout the entire bulk volume using evaporation, we employ electrokinetics to increase protein concentration to achieve supersaturated conditions favorable for crystallization within a very small localized zone inside a microchannel network. Copyright (2011) by the Chemical and Biological Microsystems Society.

published proceedings

  • 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

author list (cited authors)

  • Kim, W., Huang, Y. W., & Ugaz, V. M.

complete list of authors

  • Kim, W||Huang, YW||Ugaz, VM

publication date

  • December 2011