Label-free optical imaging of cell function and collagen structure for cell-based therapies.

abstract

• Cell-based therapies harness functional cells or tissues to mediate healing and treat disease. Assessment of cellular therapeutics requires methods that are non-destructive to ensure therapies remain viable and uncontaminated for use in patients. Optical imaging of endogenous collagen, by second-harmonic generation, and the metabolic coenzymes NADH and FAD, by autofluorescence microscopy, provides tissue structure and cellular information. Here, we review applications of label-free nonlinear optical imaging of cellular metabolism and collagen second-harmonic generation for assessing cell-based therapies. Additionally, we discuss the potential of label-free imaging for quality control of cell-based therapies, as well as the current limitations and potential future directions of label-free imaging technologies.

authors

• Walsh, Alex

published proceedings

• Curr Opin Biomed Eng

author list (cited authors)

• Hu, L., Morganti, S., Nguyen, U., Benavides, O. R., & Walsh, A. J.

citation count

• 1

complete list of authors

• Hu, Linghao||Morganti, Samantha||Nguyen, Uyen||Benavides, Oscar R||Walsh, Alex J

publication date

• March 2023

publisher

• Elsevier  Publisher

published in

• Current Opinion in Biomedical Engineering  Journal

keywords

• Collagen
• Cytotherapy
• FLUORESCENCE
• METABOLISM
• Microscopy
• Regenerative Engineering

PubMed Central ID

• 36642995

Digital Object Identifier (DOI)

• 10.1016/j.cobme.2022.100433

start page

• 100433

end page

• 100433

volume

• 25

URL

• http://dx.doi.org/10.1016/j.cobme.2022.100433