Optimizing pulse compressibility in completely all-fibered Ytterbium chirped pulse amplifiers for in vivo two photon laser scanning microscopy. Academic Article uri icon


  • A simple and completely all-fiber Yb chirped pulse amplifier that uses a dispersion matched fiber stretcher and a spliced-on hollow core photonic bandgap fiber compressor is applied in nonlinear optical microscopy. This stretching-compression approach improves compressibility and helps to maximize the fluorescence signal in two-photon laser scanning microscopy as compared with approaches that use standard single mode fibers as stretcher. We also show that in femtosecond all-fiber systems, compensation of higher order dispersion terms is relevant even for pulses with relatively narrow bandwidths for applications relying on nonlinear optical effects. The completely all-fiber system was applied to image green fluorescent beads, a stained lily-of-the-valley root and rat-tail tendon. We also demonstrated in vivo imaging in zebrafish larvae, where we simultaneously measure second harmonic and fluorescence from two-photon excited red-fluorescent protein. Since the pulses are compressed in a fiber, this source is especially suited for upgrading existing laser scanning (confocal) microscopes with multiphoton imaging capabilities in space restricted settings or for incorporation in endoscope-based microscopy.

published proceedings

  • Biomed Opt Express

author list (cited authors)

  • Fernndez, A., Grner-Nielsen, L., Andreana, M., Stadler, M., Kirchberger, S., Sturtzel, C., ... Verhoef, A.

citation count

  • 3

complete list of authors

  • Fernández, A||Grüner-Nielsen, L||Andreana, M||Stadler, M||Kirchberger, S||Sturtzel, C||Distel, M||Zhu, L||Kautek, W||Leitgeb, R||Baltuska, A||Jespersen, K||Verhoef, A

publication date

  • August 2017