Oxidative Dearomatization of PLP in Thiamin Pyrimidine Biosynthesis in Candida albicans. Academic Article

abstract

• The yeast thiamin pyrimidine synthase THI5p catalyzes one of the most complex organic rearrangements found in primary metabolism. In this reaction, the active site His66 and PLP are converted to thiamin pyrimidine in the presence of Fe(II) and oxygen. The enzyme is a single-turnover enzyme. Here, we report the identification of an oxidatively dearomatized PLP intermediate. We utilize oxygen labeling studies, chemical-rescue-based partial reconstitution experiments, and chemical model studies to support this identification. In addition, we also identify and characterize three shunt products derived from the oxidatively dearomatized PLP.

authors

• Begley, Tadhg

published proceedings

• J Am Chem Soc

altmetric score

• 11.4

author list (cited authors)

• Mondal, A., Lai, R., Fedoseyenko, D., Giri, N., & Begley, T. P.

citation count

• 0

complete list of authors

• Mondal, Anushree||Lai, Rung-Yi||Fedoseyenko, Dmytro||Giri, Nitai||Begley, Tadhg P

publication date

• March 2023

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• Candida Albicans
• Oxidative Stress
• Oxygen
• Pyrimidines
• Thiamine

PubMed Central ID

• 36802573

Digital Object Identifier (DOI)

• 10.1021/jacs.2c08560

start page

• 4421

end page

• 4430

volume

• 145

issue

• 8

URL

• http://dx.doi.org/10.1021/jacs.2c08560