This paper experimentally investigates the individual and combined effects of periodic unsteady wake flows and freestream turbulence intensity (FSTI) on flow separation along the suction surface of a low-pressure turbine blade. The experiments were carried out at a Reynolds number of 110,000 based on the suction surface length and the cascade exit velocity. The experimental matrix includes freestream turbulence intensities of 1.9%, 3.0%, 8.0%, and 13.0%, and three different unsteady wake frequencies with the steady inlet flow as the reference configuration. Detailed boundary layer measurements are performed along the suction surface of a highly loaded turbine blade with a separation zone. Particular attention is paid to the aerodynamic behavior of the separation zone at different FSTIs at steady and periodic unsteady flow conditions. The objective of the research is (i) to quantify the effect of FSTIs on the dynamics of the separation bubble at steady inlet flow conditions and (ii) to investigate the combined effects of Tuin and the unsteady wake flow on the behavior of the separation bubble.