The paper experimentally studies the effects of periodic unsteady wake flow and Reynolds number on boundary layer development, separation, reattachment, and the intermittency behavior along the suction surface of a low pressure turbine blade. Extensive unsteady boundary layer experiments were carried out at Reynolds numbers of 110,000 and 150,000 based on suction surface length and exit velocity. One steady and two different unsteady inlet flow conditions with the corresponding passing frequencies, wake velocities, and turbulence intensities were investigated. The analysis of the experimental data reveals details of boundary layer separation dynamics which is essential for understanding the physics of the separation phenomenon under periodic unsteady wake flow and different Reynolds numbers. To provide a complete picture of the transition process and separation dynamics, extensive intermittency analysis was conducted. Ensemble-averaged maximum and minimum intermittency functions were determined, leading to the relative intermittency function. In addition, the detailed intermittency analysis was aimed at answering the question as to whether the relative intermittency of a separated flow fulfills the universality criterion.