Nicotine instigates podocyte injury via NLRP3 inflammasomes activation. Academic Article uri icon


  • BACKGROUND/AIMS: Recent studies have shown that nicotine induces podocyte damage. However, it remains unknown how nicotine induces podocyte injury. The present study tested whether nicotine induces NLRP3 inflammasomes activation and thereby contributes to podocyte injury. RESULTS: Nicotine treatment significantly increased the colocalization of NLRP3 with Asc, caspase-1 activity, IL- production, cell permeability in podocytes compared to control cells. Pretreatment with caspase-1 inhibitor, WEHD significantly abolished the nicotine-induced colocalization of NLRP3 with Asc, caspase-1 activity, IL-1 production and cell permeability in podocytes. Immunofluorescence analysis showed that nicotine treatment significantly decreased the podocin and nephrin expression compared to control cells. However, prior treatment with WEHD attenuated the nicotine-induced podocin and nephrin reduction. In addition, we found that nicotine treatment significantly increased the O2.- production compared to control cells. However, prior treatment with WEHD did not alter the nicotine-induced O2.- production. Furthermore, prior treatment with ROS scavenger, NAC significantly attenuated the nicotine-induced caspase-1 activity, IL-1 production, podocin and nephrin reduction in podocytes. CONCLUSIONS: Nicotine-induced the NLRP3 inflammasome activation in podocytes and thereby results in podocyte injury. METHODS: Inflammasome formation and immunofluorescence expressions were quantified by confocal microscopy. Caspase-1 activity, IL-1 production and O2.- production were measured by ELISA and ESR.

published proceedings

  • Aging (Albany NY)

author list (cited authors)

  • Singh, G. B., Kshirasagar, N., Patibandla, S., Puchchakayala, G., Koka, S., & Boini, K. M.

citation count

  • 14

complete list of authors

  • Singh, Gurinder Bir||Kshirasagar, Naresh||Patibandla, Sai||Puchchakayala, Goverdhan||Koka, Saisudha||Boini, Krishna M

publication date

  • December 2019

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