Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords. Academic Article

abstract

• Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe invivo neuron labeling approaches, tissue-clearing technology, light sheet fluorescence microscopy, and machine learning-guided imaging analysis. This combination provides a precise determination of the cell number and somatic volume of any neuron population in the spinal cords.

authors

• Gibbs, Holly

published proceedings

• STAR Protoc

altmetric score

• 0.25

author list (cited authors)

• Yu, H., Li, Q., Sandoval, A., Gibbs, H. C., English, A., Dunn, T., ... Chen, B. o.

citation count

• 1

publication date

• December 2022

publisher

• Elsevier  Publisher

keywords

• Animals
• Cell Biology
• Image Processing, Computer-Assisted
• Mice
• Microscopy
• Microscopy, Fluorescence
• Molecular/chemical Probes
• Neurons
• Neuroscience
• Spinal Cord

Digital Object Identifier (DOI)

• 10.1016/j.xpro.2022.101759

start page

• 101759

end page

• 101759

volume

• 3

issue

• 4

URL

• http://dx.doi.org/10.1016/j.xpro.2022.101759