A novel and efficient method for identifying cotton leafroll dwarf virus infection in upland cotton (Gossypium hirsutum) Academic Article

abstract

• AbstractWe report the development of a novel and more efficient, rapid, costeffective and simple technique than current PCRbased identification methods for screening cotton (Gossypium hirsutum) plants for the presence of cotton leafroll dwarf virus (CLRDV). This protocol takes advantage of the PACE (PCR Allele Competitive Extension) system and uses PCR amplification of cDNA, coupled with sequencespecific fluorescent probes to differentiate between infected and uninfected cotton plants. This procedure has the potential for application in detection of other RNA viruses in a variety of other crops, by using primers specific for the RNAdependent RNA polymerase (RdRP) gene and a widely conserved housekeeping gene in the host organism; in this case, the G. hirsutum polyubiquitin gene (GhUB).

authors

• Alabi, Olufemi
• Stelly, David

published proceedings

• PLANT BREEDING

altmetric score

• 0.25

author list (cited authors)

• Taluja, S. M., Cheek, B. L., Vaughn, R. N., Alabi, O. J., Holme, J. E., Jain, N., & Stelly, D. M.

citation count

• 0

complete list of authors

• Taluja, Serina M||Cheek, Brianna L||Vaughn, Robert N||Alabi, Olufemi J||Holme, John E||Jain, Nisha||Stelly, David M

publication date

• April 2023

publisher

• Wiley  Publisher

published in

• Plant Breeding  Journal

keywords

• Assay Development
• Cotton
• Cotton Leafroll Dwarf Virus
• Kasp
• Pace-pcr
• Viral Detection
• Viral Testing

Digital Object Identifier (DOI)

• 10.1111/pbr.13081

start page

• 238

end page

• 246

volume

• 142

issue

• 2

URL

• http://dx.doi.org/10.1111/pbr.13081