A novel and efficient method for identifying cotton leafroll dwarf virus infection in upland cotton (Gossypium hirsutum) Academic Article uri icon


  • AbstractWe report the development of a novel and more efficient, rapid, costeffective and simple technique than current PCRbased identification methods for screening cotton (Gossypium hirsutum) plants for the presence of cotton leafroll dwarf virus (CLRDV). This protocol takes advantage of the PACE (PCR Allele Competitive Extension) system and uses PCR amplification of cDNA, coupled with sequencespecific fluorescent probes to differentiate between infected and uninfected cotton plants. This procedure has the potential for application in detection of other RNA viruses in a variety of other crops, by using primers specific for the RNAdependent RNA polymerase (RdRP) gene and a widely conserved housekeeping gene in the host organism; in this case, the G. hirsutum polyubiquitin gene (GhUB).

published proceedings


altmetric score

  • 0.25

author list (cited authors)

  • Taluja, S. M., Cheek, B. L., Vaughn, R. N., Alabi, O. J., Holme, J. E., Jain, N., & Stelly, D. M.

citation count

  • 0

complete list of authors

  • Taluja, Serina M||Cheek, Brianna L||Vaughn, Robert N||Alabi, Olufemi J||Holme, John E||Jain, Nisha||Stelly, David M

publication date

  • April 2023