Purification of human blood eosinophils by negative selection using immunomagnetic beads. Academic Article uri icon


  • A simple method for isolating highly purified eosinophils from human blood is described. Buffy coats from normal individuals (eosinophil counts less than 0.4 x 10(9)/litre) were centrifuged through a two layer Percoll density gradient, to produce a granulocyte fraction containing neutrophils and eosinophils. Neutrophils were extracted from this fraction using a monoclonal antibody (CLB FcR gran 1) against CD 16 (Fc gamma R III) in a direct or indirect selection procedure using immunomagnetic beads (Dynabeads). This negative immunoselection produced eosinophils of greater purity and with a superior capacity to mount a respiratory burst than eosinophils isolated by a method employing metrizamide.

published proceedings

  • J Immunol Methods

author list (cited authors)

  • Hansel, T. T., Pound, J. D., Pilling, D., Kitas, G. D., Salmon, M., Gentle, T. A., Lee, S. S., & Thompson, R. A.

citation count

  • 151

complete list of authors

  • Hansel, TT||Pound, JD||Pilling, D||Kitas, GD||Salmon, M||Gentle, TA||Lee, SS||Thompson, RA

publication date

  • August 1989