Purification of human blood eosinophils by negative selection using immunomagnetic beads. Academic Article

abstract

• A simple method for isolating highly purified eosinophils from human blood is described. Buffy coats from normal individuals (eosinophil counts less than 0.4 x 10(9)/litre) were centrifuged through a two layer Percoll density gradient, to produce a granulocyte fraction containing neutrophils and eosinophils. Neutrophils were extracted from this fraction using a monoclonal antibody (CLB FcR gran 1) against CD 16 (Fc gamma R III) in a direct or indirect selection procedure using immunomagnetic beads (Dynabeads). This negative immunoselection produced eosinophils of greater purity and with a superior capacity to mount a respiratory burst than eosinophils isolated by a method employing metrizamide.

authors

• Pilling, Darrell

published proceedings

• J Immunol Methods

author list (cited authors)

• Hansel, T. T., Pound, J. D., Pilling, D., Kitas, G. D., Salmon, M., Gentle, T. A., Lee, S. S., & Thompson, R. A.

citation count

• 151

complete list of authors

• Hansel, TT||Pound, JD||Pilling, D||Kitas, GD||Salmon, M||Gentle, TA||Lee, SS||Thompson, RA

publication date

• August 1989

publisher

• Elsevier  Publisher

published in

• Journal of Immunological Methods  Journal

keywords

• Antigens, Differentiation
• Cell Separation
• Centrifugation, Density Gradient
• Eosinophils
• Humans
• Immunologic Techniques
• Luminescent Measurements
• Magnetics
• Metrizamide
• Neutrophils
• Povidone
• Receptors, Fc
• Receptors, IgG
• Silicon Dioxide

PubMed Central ID

• 2547875

Digital Object Identifier (DOI)

• 10.1016/0022-1759(89)90339-6

start page

• 97

end page

• 103

volume

• 122

issue

• 1

URL

• http://dx.doi.org/10.1016/0022-1759(89)90339-6