Optimization of a Digital Mass Filter for the Isolation of Intact Protein Complexes in Stability Zone 1,1. Academic Article uri icon

abstract

  • Digital mass filters are advantageous for the analysis of large molecules due to the ability to perform ion isolation of high-m/z ions without the generation of very high radio frequency (RF) and DC voltages. Experimentally determined Mathieu stability diagrams of stability zone 1,1 for capacitively coupled digital waveforms show a voltage offset between the quadrupole rod pairs is introduced by the capacitors which is dependent on the voltage magnitude of the waveform and the duty cycle. This changes the ion's a value from a = 0 to a < 0. These effects are illustrated for isolation for single-charge states for various protein complexes up to 800 kDa (GroEL) for stability zone 1,1. Isolation resolving power (m/m) of approximately 280 was achieved for an ion of m/z 12,315 (z = 65+ for 800.5 kDa GroEL D398A), which corresponds to an m/z window of 44.

published proceedings

  • Anal Chem

altmetric score

  • 0.25

author list (cited authors)

  • Schrader, R. L., Walker, T. E., Chakravorty, S., Anderson, G. A., Reilly, P., & Russell, D. H.

citation count

  • 1

complete list of authors

  • Schrader, Robert L||Walker, Thomas E||Chakravorty, Sumeet||Anderson, Gordon A||Reilly, Peter TA||Russell, David H

publication date

  • February 2023