Microflow Cytometer for optical analysis of phytoplankton.

abstract

• Analysis of the intrinsic fluorescence profiles of individual marine algae can be used in general classification of organisms based on cell size and fluorescence properties. We describe the design and fabrication of a Microflow Cytometer on a chip for characterization of phytoplankton. The Microflow Cytometer measured distinct side scatter and fluorescence properties of Synechococcus sp., Nitzschia d., and Thalassiosira p.; measurements were confirmed using the benchtop Accuri C6 flow cytometer. The Microflow Cytometer proved sensitive enough to detect and characterize picoplankton with diameter approximately 1 m and larger phytoplankton of up to 80 m in length. The wide range in size discrimination coupled with detection of intrinsic fluorescent pigments suggests that this Microflow Cytometer will be able to distinguish different populations of phytoplankton on unmanned underwater vehicles.

authors

• Ligler, Frances

published proceedings

• Biosens Bioelectron

author list (cited authors)

• Hashemi, N., Erickson, J. S., Golden, J. P., Jackson, K. M., & Ligler, F. S.

complete list of authors

• Hashemi, Nastaran||Erickson, Jeffrey S||Golden, Joel P||Jackson, Kirsten M||Ligler, Frances S

publisher

• Elsevier  Publisher

published in

• Biosensors and Bioelectronics  Journal

keywords

• Diatoms
• Equipment Design
• FLUORESCENCE
• Flow Cytometry
• Microfluidic Analytical Techniques
• Optical Devices
• Optical Phenomena
• Phytoplankton
• Scattering, Radiation
• Species Specificity
• Synechococcus

PubMed Central ID

• 21601442

Digital Object Identifier (DOI)

• 10.1016/j.bios.2011.03.042

start page

• 4263

end page

• 4269

volume

• 26

issue

• 11