Blocking of primary in vitro antibody responses to thymus-independent and thymus-dependent antigens with antiserum specific for IgM or IgD. Academic Article

abstract

• The effect of anti-mu and anti-delta on the primary in vitro IgM response of murine splenocytes to thymus-dependent (trinitrophenylated erythrocytes) and thymus-independent (trinitrophenylated brucella) forms of trinitrophenyl was studied. The results indicate that either anti-mu or anti-delta can block the response of adult splenocytes to the thymus-dependent antigen. The thymus-dependent responses of neonatal splenocytes that bear a low concentration of IgD were also abrogated by treatment with anti-delta. In contrast, anti-mu, but not anti-delta, blocked the response of adult splenocytes to the thymus-independent antigen used. These results indicate that both IgM and IgD are receptors required for triggering cells by a thymus-dependent antigen but that only IgM receptors are required for triggering by the thymus-independent antigen used.

authors

• Ligler, Frances

published proceedings

• Proc Natl Acad Sci U S A

author list (cited authors)

• Cambier, J. C., Ligler, F. S., Uhr, J. W., Kettman, J. R., & Vitetta, E. S.

citation count

• 49

complete list of authors

• Cambier, JC||Ligler, FS||Uhr, JW||Kettman, JR||Vitetta, ES

publication date

• January 1978

publisher

• Proceedings of the National Academy of Sciences  Publisher

published in

• Proceedings of the National Academy of Sciences of the United States of America  Journal

keywords

• Animals
• Animals, Newborn
• Antibodies, Anti-Idiotypic
• Antibody Formation
• B-Lymphocytes
• Immunoglobulin D
• Immunoglobulin M
• Immunologic Capping
• In Vitro Techniques
• Mice
• Spleen
• T-Lymphocytes
• Trinitrobenzenes

PubMed Central ID

• 305047

Digital Object Identifier (DOI)

• 10.1073/pnas.75.1.432

start page

• 432

end page

• 435

volume

• 75

issue

• 1

URL

• http://dx.doi.org/10.1073/pnas.75.1.432