Utilization of microparticles in next-generation assays for microflow cytometers. Academic Article uri icon

abstract

  • Micron-sized particles have primarily been used in microfabricated flow cytometers for calibration purposes and proof-of-concept experiments. With increasing frequency, microparticles are serving as a platform for assays measured in these small analytical devices. Light scattering has been used to measure the agglomeration of antibody-coated particles in the presence of an antigen. Impedance detection is another technology being integrated into microflow cytometers for microparticle-based assays. Fluorescence is the most popular detection method in flow cytometry, enabling highly sensitive multiplexed assays. Finally, magnetic particles have also been used to measure antigen levels using a magnetophoretic micro-device. We review the progress of microparticle-based assays in microflow cytometry in terms of the advantages and limitations of each approach.

published proceedings

  • Anal Bioanal Chem

altmetric score

  • 3

author list (cited authors)

  • Kim, J. S., & Ligler, F. S.

citation count

  • 21

complete list of authors

  • Kim, Jason S||Ligler, Frances S

publication date

  • November 2010