Kinetics of antibody binding at solid-liquid interfaces in flow. Academic Article

abstract

• We have developed the theoretical framework for a displacement immunoassay conducted in flow under nonequilibrium conditions. Using a repetitive displacement technique, we determined the displacement rate and apparent dissociation rate constant at different flow rates. Our data suggest that the kinetics are best described by a first-order function. The displacement efficiency, the displacement rate, and therefore the apparent dissociation rate constant were calculated and demonstrated to be flow rate dependent. The theoretical framework developed in this study was successful in predicting the behavior of antigen displacement in flow.

authors

• Ligler, Frances

published proceedings

• J Immunol Methods

altmetric score

• 3

author list (cited authors)

• Wemhoff, G. A., Rabbany, S. Y., Kusterbeck, A. W., Ogert, R. A., Bredehorst, R., & Ligler, F. S.

citation count

• 38

complete list of authors

• Wemhoff, GA||Rabbany, SY||Kusterbeck, AW||Ogert, RA||Bredehorst, R||Ligler, FS

publication date

• December 1992

publisher

• Elsevier  Publisher

published in

• Journal of Immunological Methods  Journal

keywords

• Antibodies, Monoclonal
• Antigen-Antibody Reactions
• Immunoassay
• Kinetics
• Rheology
• Solutions

PubMed Central ID

• 1474258

Digital Object Identifier (DOI)

• 10.1016/0022-1759(92)90029-s

start page

• 223

end page

• 230

volume

• 156

issue

• 2

URL

• http://dx.doi.org/10.1016/0022-1759(92)90029-s