Surface modification and biomolecule immobilization on polymer spheres for biosensing applications. Chapter

abstract

• Microspheres and nanospheres are being used in many of today's biosensing applications for automated sample processing, flow cytometry, signal amplification in microarrays, and labeling in multiplexed analyses. The surfaces of the spheres/particles need to be modified with proteins and other biomolecules to be used in these sensing applications. This chapter contains protocols to modify carboxyl- and amine-coated polymer spheres with proteins and peptides.

authors

• Ligler, Frances

author list (cited authors)

• Taitt, C. R., Shriver-Lake, L. C., Anderson, G. P., & Ligler, F. S.

citation count

• 15

complete list of authors

• Taitt, Chris R||Shriver-Lake, Lisa C||Anderson, George P||Ligler, Frances S

Book Title

• BIOMEDICAL NANOETECHNOLOGY: METHODS AND PROTOCOLS

publication date

• January 2011

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Antimicrobial Cationic Peptides
• Biosensing Techniques
• Fumonisins
• Immunoassay
• Microspheres
• Nanotechnology
• Ochratoxins
• Polymers
• Proteins
• Surface Properties

PubMed Central ID

• 21424444

Digital Object Identifier (DOI)

• 10.1007/978-1-61779-052-2_6

International Standard Book Number (ISBN) 13

• 978-1-61779-051-5

start page

• 77

end page

• 94

volume

• 726

URL

• http://dx.doi.org/10.1007/978-1-61779-052-2_6