Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures. Academic Article uri icon

abstract

  • Integrating a perfusable microvasculature system in vitro is a substantial challenge for "on-chip" tissue models. We have developed an inclusive on-chip platform that is capable of maintaining laminar flow through porous biosynthetic microvessels. The biomimetic microfluidic device is able to deliver and generate a steady perfusion of media containing small-molecule nutrients, drugs, and gases in three-dimensional cell cultures, while replicating flow-induced mechanical stimuli. Here, we characterize the diffusion of small molecules from the perfusate, across the microvessel wall, and into the matrix of a 3D cell culture.

published proceedings

  • Biomicrofluidics

author list (cited authors)

  • Roberts, S. A., DiVito, K. A., Ligler, F. S., Adams, A. A., & Daniele, M. A.

citation count

  • 11

complete list of authors

  • Roberts, Steven A||DiVito, Kyle A||Ligler, Frances S||Adams, AndrĂ© A||Daniele, Michael A

publication date

  • September 2016