A hard microflow cytometer using groove-generated sheath flow for multiplexed bead and cell assays. Academic Article uri icon

abstract

  • With a view toward developing a rugged microflow cytometer, a sheath flow system was micromachined in hard plastic (polymethylmethacrylate) for analysis of particles and cells using optical detection. Six optical fibers were incorporated into the interrogation region of the chip, in which hydrodynamic focusing narrowed the core stream to ~35 m 40 m. The use of a relatively large channel at the inlet as well as in the interrogation region (375 m 125 m) successfully minimized the risk of clogging. The device could withstand pressures greater than 100 psi without leaking. Assays using both coded microparticles and cells were demonstrated using the microflow cytometer. Multiplexed immunoassays detected nine different bacteria and toxins using a single mixture of coded microspheres. A549 cancer cells processed with locked nucleic acid probes were evaluated using fluorescence in situ hybridization.

published proceedings

  • Anal Bioanal Chem

author list (cited authors)

  • Thangawng, A. L., Kim, J. S., Golden, J. P., Anderson, G. P., Robertson, K. L., Low, V., & Ligler, F. S.

citation count

  • 26

complete list of authors

  • Thangawng, Abel L||Kim, Jason S||Golden, Joel P||Anderson, George P||Robertson, Kelly L||Low, Vyechi||Ligler, Frances S

publication date

  • November 2010