Demonstration of four immunoassay formats using the array biosensor. Academic Article

abstract

• The ability of a fluorescence-based array biosensor to measure and quantify the binding of an antigen to an immobilized antibody has been demonstrated using the four different immunoassay formats: direct, competitive, displacement, and sandwich. A patterned array of antibodies specific for 2,4,6-trinitrotoluene (TNT) immobilized onto the surface of a planar waveguide and used to measure signals from different antigen concentrations simultaneously. For direct, competitive, and displacement assays, which are one-step assays, measurements were obtained in real time. Dose-response curves were calculated for all four assay formats, demonstrating the array biosensor's ability to quantify the amount of antigen present in solution.

authors

• Ligler, Frances

published proceedings

• Anal Chem

altmetric score

• 3

author list (cited authors)

• Sapsford, K. E., Charles, P. T., Patterson, C. H., & Ligler, F. S.

citation count

• 114

complete list of authors

• Sapsford, Kim E||Charles, Paul T||Patterson, Charles H||Ligler, Frances S

publication date

• March 2002

publisher

• American Chemical Society (ACS)  Publisher

published in

• Analytical Chemistry  Journal

keywords

• Antibodies
• Biosensing Techniques
• Environmental Pollutants
• Fluorescent Dyes
• Immunoassay
• Nasa Discipline Life Sciences Technologies
• Non-NASA Center
• Trinitrotoluene

PubMed Central ID

• 11924964

Digital Object Identifier (DOI)

• 10.1021/ac0157268

start page

• 1061

end page

• 1068

volume

• 74

issue

• 5

URL

• http://dx.doi.org/10.1021/ac0157268