An array immunosensor for simultaneous detection of clinical analytes. Academic Article

abstract

• A fluorescence-based immunosensor has been developed for simultaneous analysis of multiple samples. A patterned array of recognition elements immobilized on the surface of a planar waveguide is used to "capture" analyte present in samples; bound analyte is then quantified by means of fluorescent detector molecules. Upon excitation of the fluorescent label by a small diode laser, a CCD camera detects the pattern of fluorescent antigen:antibody complexes on the sensor surface. Image analysis software correlates the position of fluorescent signals with the identity of the analyte. This immunosensor was used to detect physiologically relevant concentrations of staphylococcal enterotoxin B (SEB), F1 antigen from Yersinia pestis, and D-dimer, a marker of sepsis and thrombotic disorders, in spiked clinical samples.

authors

• Ligler, Frances

published proceedings

• Anal Chem

altmetric score

• 9

author list (cited authors)

• Rowe, C. A., Scruggs, S. B., Feldstein, M. J., Golden, J. P., & Ligler, F. S.

citation count

• 224

complete list of authors

• Rowe, CA||Scruggs, SB||Feldstein, MJ||Golden, JP||Ligler, FS

publication date

• January 1999

publisher

• American Chemical Society (ACS)  Publisher

published in

• Analytical Chemistry  Journal

keywords

• Antigens, Bacterial
• Avidin
• Bacterial Proteins
• Biosensing Techniques
• Enterotoxins
• Fibrin Fibrinogen Degradation Products
• Fluorescent Antibody Technique
• Humans
• Image Processing, Computer-Assisted
• Immunoassay
• Nasal Mucosa
• Sensitivity And Specificity
• Time Factors
• Yersinia Pestis

PubMed Central ID

• 9949731

Digital Object Identifier (DOI)

• 10.1021/ac980798t

start page

• 433

end page

• 439

volume

• 71

issue

• 2

URL

• http://dx.doi.org/10.1021/ac980798t